Detection of SARS-CoV-2 using high-throughput PCR

Efficient management of an epidemic requires identification and isolation of infected individuals from healthypopulation to curb community spread of the pathogen. Therefore, a faster and efficient diagnostic methodfor population screening is desirable to flatten the curve of daily raise of cases. This will also help medicalprofessional to treat the epidemic efficiently. The COVID 19 pandemic caused by SARS-CoV-2 coronavirusposes a huge challenge to human being and the countries with high-density population are facing uniquechallenges. For the SARS-CoV-2 pandemic, Quantitative Real Time Polymerase Chain Reaction (qRT-PCR)emerging as a gold standard for the identification of infection; however, infrastructure requirement for thisassay is at a high-demand and therefore, resources are not abundant. We, therefore, propose a Polymerase ChainReaction (PCR)-based sensitive method for the detection of SARS-CoV-2 coronavirus. The pipeline discussedin the paper will broaden the scope of control and surveillance of COVID 19 in a high-throughput mannerwithout a sophisticated diagnostic infrastructure

A Validated Comparitative LC and Ratio First Derivative Spectrophotometric Method for the Simultaneous Determination of Levocetrizine dihydrochloride and Montelukast sodium in Bulk and Pharmaceutical dosage forms.

A new simple, rapid, precise reverse phase-high performance liquid chromatographic (RP-HPLC) and ratio spectra first derivative spectroscopy (1DD) methods has been developed for the simultaneous determination of Levocetrizine dihydrochloride (Levo) and Montelukast sodium (Mont) in bulk active pharmaceutical ingredient (API) as well as in tablet dosage form. In RP-HPLC method, separation was performed using phenomex-luna 5μ C8 (2) (100Å, 250 X 4.6 mm) column by using acetonitrile: 0.5% triethylamine in water (90:10 v/v) pH adjusted to 5.5 ± 0.1 with orthophosphoric acid. The flow rate was 0.8 ml/min with UV detection monitored at 231 nm. The retention time was 3.8 and 5.2 min for Levo and Mont respectively. In ratio spectra first derivative method, linearity range was found to be 2-32 μg/mL and 3-30 μg/mL for Levo and Mont respectively. From the first derivative (1DD) suitable wavelength was selected and amplitudes were measured at 240 nm and 281 nm for the assay of Levo and Mont by considering concentration of 18 μg/mL of Mont and 24 μg/mL of Levo as a suitable divisor, respectively. The validation of method was carried out according to ICH guidelines.